● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution.For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (SPN-C82Ek) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N were identified in the SARS-CoV-2 Mu variant (Pango lineage: B.1.621). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.