Safranin O, an indicator of cell chondrogenesis, is a cationic dye that stains acidic proteoglycan present in cartilage tissues. The Safranin O Staining Kit contains 0.1g of Safranin O Stain in powder, which can easily be dissolved in deionized water to make the staining solution. Safranin O binds to glycosaminoglycan and shows an orange-red color [1].