[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-MAADLGQIPD VDIDSDGVFK YVLIRVHLAE PSGDPAKECK
EI V R G Y KWA E Y H A DI Y DK V S GE L Q R N G Y D C E C L G G G R I S H QS Q D R K I H V Y
GYSMGYGRAQ HSVSTEKIKA KYPDYEVTWA DDGY
[ REFERENCES ]
1. Carninci P., et al. (2005) Science 309:1559-1563.
2. Kawai J., et al. (2001) Nature 409:685-690.
3. Zhang X.Q., et al. (2009) Ups. J. Med. Sci. 114:65-72.
4. Skarnes W.C., et al. (2011) Nature 474:337-342.