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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
2-(2-(Vinyloxy)ethoxy)isoindoline-1,3-dione
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
391212-30-9
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验Wash the pellet 2 times with PBS. Count the cells and resuspend to 107 cells/ml in PBS. Separation of T-Cells Mix 1 ml of AET-treated SRBC with 10 ml FBS. Mix and equal volume of PBL's with a 1% (v/v) mixture of AET-SRBC_FBS in a 50 ml tube.
Construction of homemade 'T-vectors'
against standards). 10) For ligations,use approx.25-50ng T-vector and sufficient PCR product to give a 3:1,insert:vector molar ratio(I use T4 Ligase from Gibco-BRL together with their PEG-based buffer). 11) Ligate O/N at 15°C. 12) Transform 1/5 ligation mix
mRNA Amplification with T7 RNA Polymerase
Heat oven to 37ºC. Prepare IVT Master Mix (per sample): 4 µL T7 ATP Solution 4 µL T7 CTP Solution 4 µL T7 GTP Solution 4 µL T7 UTP Solution 4 µL T7 10x Reaction Buffer 4 µL T7 Enzyme Mix Add 24 µL of IVT
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