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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
Indolizine
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
274-40-8
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验Molecular Weight Marker l Hin dIII Digest Marker Solution Digest 20 µg l DNA (40 µl DNA if at 0.5 µg/µl) Add 50 µl 10X Tracking Dye Bring volume of the digested DNA up to 500 µl
can also be excized by Cre-mediated recombination to leave a 5 base-pair duplication caused by transposon insertion plus a 274-bp insertion containing sequences encoding the 3xHA tag and the factor Xa protease cleavage recognition site. When lacZ is fused in-frame
Methods for use with the mTn-3xHA/GFP-mutagenized library
- and tetracycline-sensitive strain suitable for making plasmid preps). Select transformants with 40 ug/ml kanamycin and/or 3ug/ml tetracycline (allow at least an hour for expression following transformation). Obtain 50,000 colonies for each pool. Elute colonies
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