Recombinant Human Siglec-9 (C-6His) (CW60)
产品说明(Description)
Recombinant Human sialic acid-binding Ig-like lectin 9 is produced by our Mammalian expression system and the target gene encoding Gln18-Gly348 is expressed with a 6His tag at the C-terminus.
Accession #: AAH35365.2
Known as: Sialic acid-binding Ig-like lectin 9;Siglec-9;CDw329;Protein FOAP-9;SIGLEC9
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS,2mMEDTA,ph7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity: Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Approximately 93% will adhere after 5-10 mins incubation at 37 °C.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Sialic acid-binding Ig-like lectin 9(Siglec 9) is expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils), and found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain and so on. It is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It also binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY.