Recombinant Mouse APRIL (N-Fc)

Recombinant Mouse APRIL (N-Fc) (CB14)

产品说明(Description)

Recombinant Mouse Tumor Necrosis Factor Ligand Superfamily Member 13 is produced by our Mammalian expression system and the target gene encoding Ala96-Leu240 is expressed with a Fc tag at the N-terminus.

Accession #: AAG22534.1

Known as: A proliferation-inducing ligand;TNFSF13;TALL-2;TRDL-1;CD256

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control) 

 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity-BLI:
Loaded Human BCMA-His(Cat#CC28) on HIS1K Biosensor, can bind  Mouse APRIL-Fc(Cat#CB14) with an affinity constant of <10-3 nM as determined in BLI assay.		Loaded Human BCMA-His(Cat#C01B) on HIS1K Biosensor, can bind  Mouse APRIL-Fc(Cat#CB14) with an affinity constant of 0.14 nM as determined in BLI assay.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

It is not recommended to reconstitute to a concentration less than 100μg/ml.

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

 

Tumor necrosis factor ligand superfamily member 13 belongs to the tumor necrosis factor family. It is also known as APRIL, TALL2, TRDL1, and CD256. It is synthesized as a 32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule. TNFSF13 is a Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA and plays a role in the regulation of tumor cell growth. It expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages. Its expression by CD4+ T cells inhibits the production of Th2 cytokines and allergic inflammation.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY