Recombinant Human tPA/Tissue-t

Recombinant Human tPA/Tissue-type plasminogen activator/PLAT  (CX67)

 

产品说明(Description)

Recombinant Human Tissue-type plasminogen activator is produced by our Mammalian expression system and the target gene encoding Ser36-Pro562 is expressed with a 6His tag at the C-terminus. 

Accession #: P00750

Known as: T-PA; TPA; t-plasminogen activator; Tissue plasminogen activator;

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 5% Trehalose, 0.2mM CaCl2, pH 5.5.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Tissue-type plasminogen activator (PLAT) is a protein that secreted  into extracellular space. PLAT contains five domains: EGF-like domain, fibronectin type-I domain, 2 kringle domains and peptidase S1 domain. It belongs to the peptidase S1 family. The main function of this protein is to convert plasminogen into biologically active plasmin. As a protease, PLAT plays a crucial role in regulating blood fibrinolysis, maintaining the homeostasis of extracellular matrix and in modulating the post-translational activation of growth factors. PLAT is found not only in the blood, where its primary function is as a thrombolytic enzyme, but also in the central nervous system (CNS). It participates in a number of physiological and pathological events in the CNS, as well as the role of neuroserpin as the natural regulator of PLAT's activity in these processes. Increased or decreased activity of PLAT leads to hyperfibrinolysis or hypofibrinolysis, respectively. In addition, as a cytokine, PLAT plays a pivotal role in the pathogenesis of renal interstitial fibrosis through diverse mechanisms. Thus, as a fibrogenic cytokine, it promotes the progression of kidney diseases.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY