Recombinant Human FAP (N-8His)

Recombinant Human FAP (N-8His) (C14G)

产品说明(Description)

Recombinant Human Prolyl endopeptidase FAP is produced by our Mammalian expression system and the target gene encoding Leu26-Asp760 is expressed with a 8His tag at the N-terminus.

Accession #: Q12884

Known as: FAP; FAPA; DPPIV; SIMP; Fapalpha

 

制剂(Formulation)

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 20% Glycerol, pH 8.0.

 

质量控制(Quality Control)

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

Bioactivity-ELISA:		Bioactivity-BLI:
Immobilized Human FAP -His(Cat#C14G) at 2μg/ml (100 μl/well)can bind Anti-Human FAP mAb (Cat#NC073). The ED50 ofAnti-Human FAP mAb (Cat#NC073)is 13.69 ng/ml.		Loaded Anti-Human FAP mAb(Cat#NC073) on Pro A Biosensor, can bind Human FAP-His(Cat#C14G) with an affinity constant of <10-3 nM as determined in BLI assay.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.   
 

背景(Background)

FAP (also known as seprase) is a single-pass type II membrane protein which belongs to thepeptidase S9B family. FAP appears to act as a proteolytically active 170-kDa dimer, consisting of two 97-kDa subunits. It is a member of the group type II integral serine proteases, which includes dipeptidyl peptidase IV ( DPPIV /CD26 ) and related type II transmembrane prolyl serine peptidases, which exert their mechanisms of action on the cell surface. FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin as well as several peptide hormones. The enzymatic activity is dependent on FAP association with DPPIV on the cell surface. The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion. In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY