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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
咨询
- 英文名:
Vitamin A Acetate
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
127-47-9
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验at 4 C. Periodically dump the 1X TE and make up the fresh 1X TE from 10X stock. 10X TE (10:1) 1X 1L 1X 1L 10X 10mM Tris-HCl 1.06 g Tris-HCl 10.6 g 0.39 g Tris-Base 3.94 g 1mM Na2EDTA 0.475 g 4.75 g pH 8 at 25 C. Mix and store at 4 C
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别
1.2 = 0.96 M 22.694 g Ammonium thiocyanate 0.4 M x 1.2 = 0.48 M 7.308 g Sodium Acetate 0.1 M x 1.2 = 0.12 M, pH 5 Stock 1M 24 ml Glycerol 5% x 1.2 = 6% 12ml Add water to 100ml Mix well Filter Add phenol 76 ml x 1.2 = 91.2 and mix. Phenol
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别提取细胞核和细胞质RNA
concentration of phenol is 38%. Add water to 200 ml total volume. pH ~ 4.5. Wrap with aluminum foil. 1.2xTRIzol: 200ml Final Add Guanidine thiocyanate 0.8 M x 1.2 = 0.96 M 22.694 g Ammonium thiocyanate 0.4 M x 1.2 = 0.48 M 7.308 g Sodium Acetate 0.1 M
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