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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
咨询
- 英文名:
Silica gel 300-400M
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
112926-00-8
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
50% glycerol100 mM Tris-HCl pH8 @ 25 degrees 100 microliters 1 M Tris pH8300 mM KCl 300 microliters 1 M KCl25 mM MgCl2 25 microliters 1 M MgCl2500 micrograms/ml BSA 5 microliters 100 mg/ml BSA170 microliters H2Ooptional: add 25 microliters saturated
2-D Polyacrylamide Gel Electrophoresis
O,under the hood . 2.Stir to dissolve. 3.Bring volume to 1 L. 4.Filter through 0.45 µm pore size filter. 5.Store at 4℃ in the dark.D: Separating Acrylamide Gel Below are the solution volumes required to prepare one 9-18% gradient gel.Prepare sufficient
Native Chromatin Preparation and Illumina/Solexa Library Construction
. Elute with 20-25 µL of buffer EB. 44. Perform size selection: i. Load 20 µL of linker-ligated DNA onto a 2% E-Gel and perform electrophoresis. ii. Cut the gel around the 200-400 bp region
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