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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
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- 英文名:
Methotrexate xhydrate
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
133073-73-1
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验on a hemocytometer. Cell Fusion The ideal cell ratio of spleen to tumor cells is 5:1. Fuse a maximum of 1.5 - 2.5 x 108 spleen cells per tube. If fusing a larger number of spleen cells, divide them into two tubes. Mix the appropriate volumes
ES Cell Culture and Manipulation
sure that the concentration is know. Preparation of cells for transformation For one electroporation experiment you will need approximately 6 x 107 ES cells (~3 confluent 10cm plates). Feed the cells in the morning and wait a couple
ES Cell Culture and Manipulation
of cells for transformation For one electroporation experiment you will need approximately 6 x 107 ES cells (~3 confluent 10cm plates). Feed the cells in the morning and wait a couple hours before electroporating. Trypsinize the cells
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