2,4,6-三氯苯酰氯,2,4,6-Trichloroben

F值表（方差齐性检验用）

3.58 3.41 3.29 3.20 3.12 3.06 2.96 2.86 2.76 2.64 2.52 2.39 15 16 4.69 4.08 3.73 3.50 3.34 3.22 3.12 3.05 2.99 2.89 2.79 2.68 2.57 2.45 2.32 16 17 4.62 4.01 3.66 3.44 3.28 3.16 3.06 2.98 2.92 2.82 2.72 2.62 2.50 2.38 2.25 17 18 4.56 3.95

Assessment of Magnaporthe grisea mating type by spore PCR

) containing 50 mM KCl, 0.1 Triton X-100, 2.5 mM MgCl2, 0.2 mM each dNTP, 1.0 mM each primer, 1.25 units of Taq DNA polymerase (Promega). Samples are overlaid with 50 ul mineral oil and subjected to the following PCR condition: 95 C for 5 min; 30 cycles of 95 C

Methods for DNA isolation

in cesium chloride-ethidium bromide gradients (1). Briefly, cells containing the desired plasmid or cosmid are harvested by centrifugation, incubated in a lysozyme buffer, and treated with alkaline detergent. Detergent solubilized proteins and membranes