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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
Methyl 2-bromoimidazo[1,2-a]pyridine-6-carboxylate
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
1042141-37-6
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验Two dimensional peptide mapping
(enough to cover the piece of membrane). Start the digestion by adding 10 micrograms trypsin or chymotrypsin (Stock is 1 milligram/ml in 0.05 M NH4HCO3 stored at -70°C) . Mix by pipetting up and down and incubate at 37°C for 2 hr. 5. Vortex the sample
Phosphoamino acid analysis:Mark Kamps's method
of each buffer. pH 1.9 buffer. 88% Formic acid 50 ml Acetic acid 156 ml H2O 1794 ml Don't use the 98% formic acid and don't adjust the pH. pH 3.5 buffer Pyridine 10 ml Acetic acid 100 ml 100 mmolar EDTA 10 ml H2O 1880 ml Don't bother to adjust
2 CO 3 ) 5% citric acid Isobutylchloroformate (98%) 100‐, 20‐, 10‐, 5‐, and 1‐mL
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