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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
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- 英文名:
Copper Pyrophosphate
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
10102-90-6
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验Two dimensional peptide mapping
from the protease adsorbing onto the filter. 3. Aspirate the liquid. Wash the membrane extensively with H2O (5 X 1ml). Then wash with freshly-made 0.05 M NH4HCO3 once or twice. 4. Digestion: Add 150 -200 microliter fresh 0.05 M NH4HCO3
Northern Blotting步骤和试剂配制(Breeden Lab)
) and without non-homologous DNA 100 x Denhardt"s solution: [for 500 ml: 10 g Ficoll 400; 10 g polyvinylpyrrolidone MW 360000; 10 g BSA fraction V; H2O] store at -20 °C. 20 x SSC: 3 M NaCl; 0.3 M Na-citrate [1 l: 175.3 g NaCl, 88
Replication timing by density transfer
volume of a 2x enzyme mix that contains the appropriate restriction enzyme buffer, restriction enzyme (usually 1 microliter per sample) and 0.25 microgram/ml RNase A. If the DNA prep is clean, the digest can go overnight at 37 degrees C.] Choosing
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