环.己.酮肟(Chunks or pellets) ,Cyc

Methods for DNA isolation

will add 100 ml TE-RNase solution to the cell pellets and mix to partially resuspend. 7. Next, the biomek will add 100 ml alkaline lysis solution to the wells of the filter plate. 8. The biomek then will mix the cell suspension again, transfer

C. elegans whole mount immunohistochemistry with Bouin Fixative

for many months). Nematodes grown on NGM plates (usually from chunks for 2 days at 22.5 °C) are washed off the plate using 2 to 3 ml of H2O and spun down in a glass 12 ml conical tube in a clinical centrifuge at RT (approximately 23°C in my lab). The supernatant

Replication timing by density transfer

4 , 2.0 M NaCl, pH 6.8 T10 E100 Add to 350 ml dH2 O: 18.61 g Na2 EDTA?H2 O 1.5 g NaOH pellets 5 ml 1M Tris-HCl pH 7.5 Stir until the solids have dissolved, bring the pH to 7.5 with concentrated NaOH (2 O