二辛基亚砜,Di-n-octylsulfoxide,98%,

（共享）免疫组织化学染色FAQ

.), which does not seem to have been used as a fixative. Carbodiimides are compounds that combine with and cross-link carboxyl groups. They fix proteins by joining together C-termini and/or side chains of glutamic and aspartic acid units. Their general chemical formula is R-N=C=N-R

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7.0 ) ，1~5 mmol/L  X-Gluc(X- Gluc 可以用二甲基亚砜溶解，Sigma)。3. 注释 注 1 : 种子还可用 70% 乙醇消毒 2 min；用无菌蒸馏水洗一次，然后在 50%  Clorox 漂白剂中浸泡 15 min，期间以 0.5 g 持续振荡。 注 2 : 用灭菌的滤纸汲取种子上的水分（3 mm；VWR) ，再将消毒后的种子放置于 MS1 培养基上。 注 3 : 芽尖包含有顶端分生组织、2~3 个叶原基和叶基。芽尖是靠近叶基的膨大部分。 注 4 : 每一品种放置

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tumor GH3 and human promyelocytic leukemia HL-60 cells. Arch. Biochem. Biophys.249 (1986) 148-153. [7]Soldatenkov, V.A. and Potaman, V.N. DNA-binding properties of poly(ADP-ribose)polymerase: a target for anticancer therapy. Curr. Drug Targets 5 (2004