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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
咨询
- 英文名:
Deoxyribonuclease I
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
9003-98-9
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验so that there are no clumps of cells. Incubate on ice for 10 minutes;Centrifuge at 5,000 rpm for 5 minutes at 4°C to pellet the nuclei;Re-suspend nuclei in 600µl of ChIP lysis buffer+1/1000 PMSF+50 units of RNAse inhibitor and incubate on ice for 10 minutes; Sonicate
by bivalent metals. Maximum activation is attained with Mg+ and Ca+. Dnase I is inhibited by EDTA and SDS. The optimum pH of Deoxyribonuclease I from Bovine Pancreas is 7.8. 2.1.4. What is an acceptable enzyme diluent for Elastase (ES)? Elastase
Supplies: Ethanol 100 USP (highest quality) FACS Staining Buffer (1XPBS w/ 3 calf serum, 0.05 azide--filtered) ―Dilute staining antibodies in Buffer DNAse (Sigma D-5025, Bovine Pancreas) RNase (Boehringer, 25 mg bovine pancrease)
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