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- 文献和实验
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- 英文名:
1,1-Bis(4-(diethylamino)phenyl)prop-2-yn-1-ol
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
228415-25-6
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
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文献和实验Preparation of Polyacrylamide Gels
2.5 g Bis-Acrylamide 8 M Urea 422.0 g Urea 0.5 X TBE 25 mL 20X TBE 500 mL ddH20 Mix. Bring volume to 1 L. Filter. Store at 4 C in the dark. 3. Wash glass plates on both sides with soap. Rinse well with water. Rinse well with distilled water
Reverse Ligation Mediated RT -
Sequencing gel loading buffer (95% formamide, 10mM NaOH, 0.05% bromophenol blue, 0.05% xylene cyanol) Sequencing gel mix (6 - 8% polyacrylamide : bis (19 : 1), 7M urea, 1x TBE buffer) Sequencing Grade Taq + 5x reaction buffer ( Promega ).
Reverse Ligation Mediated RT - PCR
primer (DigP). Sequencing gel loading buffer (95% formamide, 10mM NaOH, 0.05% bromophenol blue, 0.05% xylene cyanol) Sequencing gel mix (6 - 8% polyacrylamide : bis (19 : 1), 7M urea, 1x TBE buffer) Sequencing Grade Taq + 5x
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