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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
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- 英文名:
Tin(II) Acetate
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
638-39-1
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验Crystallization of Kinesin Family Motor Proteins
parameters.Crystals of monomeric rat Kinesin-1Table 1: Crystallization conditions for kinesin motor protein constructsConstructMethodConditionsReferencesHuman Kinesin-1 hK349Sitting drop 4°C5 mg/ml protein in 50 mM Na-acetate, pH 4.6, 75 mM KCl, 3.5% (w/v
and ethanol precipitation:• Phenol:chloroform:isoamyl alcohol (25:24:1; See Sambrook et al., 1989, for information onpreparing neutralized phenol solutions)• 10 M ammonium acetate• 95–100% ethanol1.Prepare yeast cultures for lysis (Step a, b, or c
volume to 40 µl. Add 60µl of the eluate from the purification of labeled primer. Add 10µl (1/10 volume) of 3M sodium acetate. Add 250µl of 95% ethanol. Incubate overnight at -70°C. Spin for 30 minutes at full speed. Remove
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