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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
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- 英文名:
1-Butyl-3-vinyl-1H-imidazol-3-ium bromide
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
34311-90-5
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验James Hardwick CNBr Cleavage Procedure
tube containing 200 microliters of 100mg/ml CNBr in 70% formic acid. CNBr stocks are 200 milligrams/ml in 70% formic acid and are stored at -70°C. 70% formic acid is made from 98-100% formic acid and MQH2O. Optional: If you want to know
E.Z.N.A. Cycle-Pure Kit Spin Protocol
步骤 1. Perform agarose gel/ethidium bromide electrophoresis to analyze PCR product. 2. Determine the volume of the PCR reaction, transfer the sample into a clean 1.5ml microcentrifuge tube, and add 4-5 volumes of Buffer CP. For PCR
Replication timing by density transfer
, then add Pronase (0.01-0.05 mg/ml final conc.). [You can add dry Pronase. I usually dissolve the Pronase in 5 ml of minimal medium, just to avoid having the dry Pronase stick to the sides of the flask.] 5. Hold at 37o C for 90-120 min
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