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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic peptide within human Retrovirus-related Gag aa 193-236.
- 亚型:
IgG
- 形态:
Liquid
- 克隆性:
Rabbit polyclonal Antibody
- 标记物:
Non-conjugated
- 库存:
现货
- 供应商:
华安生物
- 宿主:
Rabbit
- 应用范围:
ICC
- 浓度:
1 mg/mL.
- 规格:
50ul/100ul
| 规格: | 50ul | 产品价格: | ¥1125.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1875.0 |
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文献和实验Material: Packaging Cells, e.g. Phoenix cells (an adenovirus Ad5-transformed human embryonic kidney cell line 293T, transfected with two MoMLV packaging gene constructs: CMV-Env-PolyA, and RSV-Gag/Pol-Tyt2-PolyA.) See the Nolan lab
before infection set up J2-3T3s in 6-well plates We use J2-3T3s because they give nice discreet colonies after selection They want to be 20-80% confluent on the day of infection Some retrovirus cannot be titred on rodent cells such as 3T3. For example
Lineage Analysis Using Retrovirus Vectors
that encodes the structural proteins. Thus, the packaging lines, or cells transfected with packaging constructs, make viral particles that do not contain the genes gag, pro, pol, or env . Retrovirus vector particles are essentially
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