Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
克隆性:
Rabbit polyclonal Antibody
标记物:
HRP-conjugated
适应物种:
Rat
库存:
现货
供应商:
华安生物
宿主:
Rabbit
应用范围:
WB, ELISA
浓度:
2 mg/ml.
规格:
100 μl/500 μl/1ml
规格:
100 μl
产品价格:
¥170.0
规格:
500 μl
产品价格:
¥595.0
规格:
1ml
产品价格:
¥1020.0
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.