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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
咨询
- 保质期：
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- 英文名：
3-Bromo-5-phenoxypyridine
- 库存：
咨询
- 供应商：
无锡云萃生物
- CAS号：
28232-63-5
- 规格：
5mg/50mg/100mg/500mg
| 规格： | 5mg | 产品价格： | ￥100.0 |
|---|---|---|---|
| 规格： | 50mg | 产品价格： | ￥100.0 |
| 规格： | 100mg | 产品价格： | ￥100.0 |
| 规格： | 500mg | 产品价格： | ￥100.0 |
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文献和实验Methods for use with the mTn-lacZ/LEU2-mutagenized library
. Screening 30, 000 transformants should give you 95% coverage of the yeast genome. To minimize double integrants, transformations should contain the lowest amount of DNA practicable. We therefore recommend that a pilot experiment be performed
1/2 volume of 1-bromo-3-chloro-propane (molecular biology grade, SIGMA) as chloroform substitute. For efficient recovery of small RNAs, DNA LoBind tubes (Eppendorf) were used and all centrifugation steps were performed at maximum speed and 4°C in an Eppendorf 5417R
Methods for use with the mTn-3xHA/lacZ-mutagenized library
was made from a circle-zero strain (also rho-minus) so this is not necessary. Screening 30, 000 transformants should give you 95% coverage of the yeast genome. To minimize double integrants, transformations should contain the lowest amount of DNA practicable
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