4-溴-3-羟基噻吩-2-羧酸甲酯,Methyl 4-bro

mTn-3xHA/lacZ

to the gene of interest, the excision event results in an in-frame insertion of 93 amino acids, called a HAT tag, into the encoded protein. Insertion of the HAT tag has the potential to create conditionally-defective forms of the protein.   GenBank

TLC显色试剂及配方大全

化铁／磺基水杨酸 检出物：硫代磷酸酯类。 溶液：I．1％氯化铁的80％乙醇溶液；Ⅱ．1％磺基水杨酸的80％乙醇溶液， 方法：薄层先置溴蒸气中10min后，喷溶液I，干燥15min，再喷溶液Ⅱ。 结果：紫色背景上呈白色。 ④叠氮化碘 检出物：含硫氨基酸、硫化物与青霉素。 溶液：叠氮碘溶液-叠氮钠3g 溶乎100ml 0.05mol／L碘溶液中(新鲜配制，固体叠氮碘有爆炸性)。 ⑤靛红／硫酸 检出物：噻吩衍生物。 溶液；0.4g靛红溶于100ml浓硫酸

Methods for use with the mTn-3xHA/lacZ-mutagenized library

was made from a circle-zero strain (also rho-minus) so this is not necessary. Screening 30, 000 transformants should give you 95% coverage of the yeast genome. To minimize double integrants, transformations should contain the lowest amount of DNA practicable