O-(4-联.苯甲酰基)苯甲酸,o-(4-Biphenyly

Proteolytic Labeling With 18O for Comparative Proteomics Studies: Preparation of 18O-Labeled Peptides and the 18O/16O Peptide Mixture

and redissolved in H2 18 O for the labeling reaction. Incorporation can exceed 95% if it is carried out in water that is sufficiently enriched with H2 18 O. The byproduct of the reaction is water. The use

Structural Determination of O-Glycans by Tandem Mass Spectrometry

Trans -sialidase (TS; E.C. 3.2.1.18) catalyzes the transfer of preferably α2,3-linked sialic acid to another glycan or glycoconjugate, forming a new α2,3-linkage to galactose or N -acetylgalactosamine. In the absence of an appropriate

Identification of Asp Isomerization in Proteins by 18O Labeling and Tandem Mass Spectrometry

to identify the site of modification by LC-MS/MS peptide mapping. Here, we describe an approach to label the Asp residue involved in isomerization at the protein level by hydrolyzing the succinimide intermediate in H 2 18 O. Tryptic digestion of this labeled