5-羟甲基.噻.吩-2-硼酸,(5-(Hydroxymeth

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

) and Cs2 CO3 under a nitrogen atmosphere. Conversion to the O 6 ?(benzotriazol?1?yl) ethers occurs within about 10 min for inosine, and within about 60 min for guanosine and 2??deoxyguanosine. Then, for reaction with alcohols, the reaction mixture

单组分溶液配制

. Buffer Mw pKa 20o C Working range delta pKa per 10o C MES 2-(N -morpholino)ethanesulfonic acid 195.2 6.15 5.5-6.7 -0.110 Bis-Tris bis (2-hydroxyethyl)iminotris (hydroxymethyl)methane 209.2 6.5 5.8-7.2   ADA N -(2-acetamido

【原创】非变性聚丙烯酰胺凝胶电泳分离大鼠海马组织蛋白

Bisacrylaminde,Bis），过硫酸铵（Ammonium Persulfate,AP），四甲基乙二胺（TEMED），三羟甲基氨基甲烷（hydroxymethyl aminomethane,TRIS），考马斯亮兰R250（Coomassie Brilliant Blue R250），乙二胺四乙酸二钠（EDTA）等试剂进口分装，购自上海实生细胞生物技术有限公司 1.3 溶液配制 1.3.1样品提取液[3]：0.15mol/L NaCl，0.005mol/L EDTA，10mmol/LKCl，1.5mmol