3-苄氧基苯硼酸,(3-(Benzyloxy)phenyl)

硅胶基硝化苯硼酸亲和色谱材料的合成及应用

of Chemical Physics,Chinese Academy of Sciences, Dalian 116023） 　　Abstract  A boronic acidsubstituted silica was synthesized by using a γglycidoxypropyltrimethoxysilane as the spacer arm and using a 3amino4nitrobenzeneboronic acid as the ligand. The 3amino

应用末端截切、进化、再延长技术提高酶稳定性的方法

/Cm：2YT 培养基中加入 25 μg/ml 氯霉素。 ( 10 ) 高速离心机（Sorvall、GS-3 和 SS -34 转子）。 ( 11 ) 悬浮缓冲液：50 mmol/L 磷酸钠、500 mmol/L NaCl，pH 7.0。 ( 12 ) Benzonase ( Sigma)。 ( 13 ) 0.45 μm 磺酸多醚注射过滤器。 ( 14 ) 2 ml 苯硼酸柱子。 ( 15 ) 硼酸缓冲液：0.5 mol/L 硼酸、0.5 mol/L NaCl

Synthesis of Triazole‐Nucleoside Phosphoramidites and Their Use in Solid‐Phase Oligonucleotide Synthesis

of two triazole?nucleoside phosphoramidites [uracil?triazole?uracil (UtU) and cytosine?triazole?uracil (CtU)] based on a PNA?like scaffold, and their incorporation within oligonucleotides. Curr. Protoc. Nucleic Acid Chem . 55:4.57.1?4.57.38. © 2013 by John Wiley