2,6-二氟-3-硝.基.苯硼酸,(2,6-Difluoro

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of Chemical Physics,Chinese Academy of Sciences, Dalian 116023） 　　Abstract  A boronic acidsubstituted silica was synthesized by using a γglycidoxypropyltrimethoxysilane as the spacer arm and using a 3amino4nitrobenzeneboronic acid as the ligand. The 3amino

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/Cm：2YT 培养基中加入 25 μg/ml 氯霉素。 ( 10 ) 高速离心机（Sorvall、GS-3 和 SS -34 转子）。 ( 11 ) 悬浮缓冲液：50 mmol/L 磷酸钠、500 mmol/L NaCl，pH 7.0。 ( 12 ) Benzonase ( Sigma)。 ( 13 ) 0.45 μm 磺酸多醚注射过滤器。 ( 14 ) 2 ml 苯硼酸柱子。 ( 15 ) 硼酸缓冲液：0.5 mol/L 硼酸、0.5 mol/L NaCl

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.D., Kettner, C.A., Petsko, G.A., Fuller, R.S., and Ringe, D. 2003. 2.4 Å resolution crystal structure of the prototypical hormone‐processing protease kex2 in complex with an ala‐lys‐arg boronic acid inhibitor. Biochemistry 42:6709‐6718