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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic peptide within Human FKBP12 aa 76-108 / 108.
- 亚型:
IgG
- 形态:
Liquid
- 克隆性:
Recombinant Rabbit monoclonal Antibody
- 标记物:
Non-conjugated
- 适应物种:
Human,Mouse
- 库存:
现货
- 供应商:
华安生物
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF
- 浓度:
1 mg/mL.
- 规格:
50μl/100μl
| 规格: | 50μl | 产品价格: | ¥1125.0 |
|---|---|---|---|
| 规格: | 100μl | 产品价格: | ¥1875.0 |
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文献和实验Ligand-Regulated Peptide Aptamers
, we have developed a peptide aptamer scaffold protein that can impart small-molecule control over the aptamer–target interaction. This ligand-regulated peptide (LiRP) scaffold, consisting of the protein domains FKBP12, FRB, and GST, binds to the cell-permeable small
) and GST-FRB and (His)6-FKBP12 were produced recombinantly in Escherichia coli. IKBalpha and (His)6-FKBP12 were labeled with Cy3 and Cy5, respectively, using monofunctional reactive dye from Amersham Pharmacia Biotech (Newark, NJ) and following
生物公司), 37℃条件下放置1h 6、用PBS冲洗3次,每次10min,最后在倒置荧光显微镜(Leica公司)下观察图像并拍照。 200× 400× 三、Real-time PCR检测SD大鼠心房肌细胞CaMK-Ⅱδ、A基因、B基因、FKBP12.6 mRNA表达水平 1、总RNA的提取: (1)将细胞培养液吸出后,用PBS洗细胞2次,加入1 mL Trizol室温裂解细胞5min,用移液器均匀吹下细胞并置于EP管中,上下轻柔颠倒10次,室温静置5min (2)加0.2
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