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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against synthesized peptide derived from human AOX1. AA range:521-570
- 亚型:
IgG
- 形态:
Liquid
- 克隆性:
Rabbit polyclonal Antibody
- 标记物:
Non-conjugated
- 适应物种:
Human, Rat
- 库存:
现货
- 供应商:
华安生物
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P
- 浓度:
1 mg/mL.
- 规格:
50μl/100μl
| 规格: | 50μl | 产品价格: | ¥1125.0 |
|---|---|---|---|
| 规格: | 100μl | 产品价格: | ¥1875.0 |
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文献和实验The Generation of Multicopy Recombinant Strains
The extremely high levels of alcohol oxidase produced from the native AOX1 gene in Pichia pastoris (5–30% of cell protein on induction) suggested that single-copy AOX1 -promoter expression vectors would be sufficient for efficient foreign gene
High Cell-Density Fermentation
(AOX1 ) promoter for high-level expression of foreign genes. The AOX1 promoter is induced by methanol, but repressed in the presence of excess glycerol. Glycerol is readily metabolized by P. pastoris , and high-cell densities can be obtained utilizing
和Pichia 2个种属,共有30余种[8],其中尤以P.pastoris发展最快,研究最清楚。目前,用于外源基因表达的P.pastoris菌株为Invitrogen公司构建,主要有:Y11430,MG1003,GS115,KM71和SMD1168,其中Y11430为野生型,其余皆为组氨酸突变型,GS115有完整的aox1基因,在甲醇培养基上表型为Mut+,当用重组表达载体转化后,由于表达载体线化所用酶不同,其转化子表型可能是Mut+或Muts,可通过MD和MM培养基进行表型的鉴定;KM71的aox1
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