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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
tert-Butyl (2-bromophenyl)carbamate
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
78839-75-5
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验In vitro transcription with yeast nuclear extract
till bromophenyl blue is 2-3 inches from the bottom of gel. Primer Annealing mix (for 1 reaction (10 microliters)) 2 microliters 5x annealing buffer~0.15 microliters 32P kinased lacI oligo (typically 1-5 x 105 cpm from 0.5 microgram kinased oligo resuspended
In vitro transcription with yeast nuclear extract
sequencing gel and run till bromophenyl blue is 2-3 inches from the bottom of gel. Primer Annealing mix (for 1 reaction (10 microliters)) 2 microliters 5x annealing buffer ~0.15 microliters 32P kinased lacI oligo (typically 1-5 x
Invitro transcription with yeast nuclear extract
throughout, use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and pipetmen with 95% ethanol before use to eliminate any stray RNAses . 1. For a 25 microliter transcription reaction, add the following to a microfuge tube
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