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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
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- 英文名:
2-(2-Bromoethoxy)tetrahydro-2H-pyran
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
17739-45-6
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
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文献和实验DNA ISOLATION FROM PRIMARY TUMORS VIA CRYOSECTIONS
- make a 5µm section to do an evaluation of the % tumour cells - make 50X50µm sections for the DNA isolation - put sections in a falcon tube containing 10 ml of 1XSE - add 100µg/ml prot. K (endconcentration) (1 ml of 1mg/ml in 10 ml of SE buffer
多核糖体mRNAs是酿酒酵母中mRNA无义介导降解途径的底物
nonsense-containing transcripts of the LEU2, HIS4, PGK1, and CYH2 genes, and two wild-type mRNAs (the MAT(alpha)1 and CYH2 mRNAs), were stabilized when the translation elongation inhibitor cycloheximide was added to cellular growth media. Subsequent removal
DNA ISOLATION FROM PRIMARY TUMORS VIA CRYOSECTIONS
100µg/ml prot. K (endconcentration) (1 ml of 1mg/ml in 10 ml of SE buffer) + 1% SDS (endconcentration) (stock is 25% SDS) - put the tube o/n at 55°C - add 1/4 of the volume (2.5 ml) of 6M NaCl(saturated solution, precipitation
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