邻菲啰啉盐酸盐,1,10-Phenanthroline hy

Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper

The existence of cell-type specific promoter and enhancer elements has been known for several years. However, the mechanisms responsible for the remarkable specificity of such elements, in comparison to the ubiquitously active promoters

质疑｜NEJM 疑似误用「尿结晶」图片，医学顶刊也犯低级错误？！

甲基异恶唑结晶 [7] ，如图 3图 3 还有非典型的磺胺甲基异恶唑结晶，如图 4：图 4图源：Daudon 和 Frochot 博士N - 乙酰磺胺甲基异恶唑盐酸盐结晶类似于胱氨酸结晶或草酸钙结晶 [8]。如下图 5，易与胱氨酸结晶相混淆，通过相差和偏振光（插图）可以看到 N - 乙酰磺胺甲基异恶唑盐酸盐六边形结晶，右图可见薄板状的 SMX 结晶，在偏振光显微镜下，N - 乙酰磺胺甲基异恶唑盐酸盐六边形结晶具有很强的双折射和多色性 [8]。令人遗憾的是，偏振光设备仅在约 10% 的临床实验室可用

SINGLE STEP PROKARYOTIC RNA ISOLATION

2-mercaptoethanol        10 mM           4.4 mM 5% SDS - 1 gram per 20ml 2.3 mg/ml Protease K - 46 mg in 20ml ddH2O 0.1M EDTA - 4ml 0.5M EDTA + 16ml ddH2O 20mM 1,10-phenanthroline - 79 mg in 20ml ddH2O(boil to dissolve) 2 mg/ml Heparin - 40 mg in 20ml ddH