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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
咨询
- 英文名:
3-Bromo-5-methoxypyridine
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
50720-12-2
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验dNTP量=[(0.4nmol/μl×100μl)-0.48nm]×1.18% =0.47nmol 合成第二链cDNA量(ng)=0.47nmol×330ng/nmol =155ng 双链cDNA转变率=155ng /158ng×100%=98% 一般cDNA第一链转变率和双链转变率以12-50%和50-200%为好。 (四) 电泳分析 通常合成的cDNA第一链和第二链长度为350-6000碱基,需进行1.4%碱性琼脂糖电泳。将第一链和第二链掺入测定管中的反应液先用酚抽提,乙醇
Methods for use with the mTn-3xHA/lacZ-mutagenized library
Making library DNA from the DNA we send you The original library was distributed as 18 individual pools. After 8/98 , there is a new library that is distributed as 10 pools. You will be sent about a microgram
, without mercuric chloride? [ B-5 is: Water 90 ml Formalin (40% HCHO) 10 ml Mercuric chloride 6 g Sodium acetate (anhydrous) 1.25 g The sodium acetate brings the pH into the 5.8-6.0 range. Fix by immersion, 12-24 hours, then transfer to 70-80% alcohol
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