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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
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- 保质期：
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- 英文名：
(E)-Methyl 4-(4-bromophenyl)-2-oxobut-3-enoate
- 库存：
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- 供应商：
无锡云萃生物
- CAS号：
608128-34-3
- 规格：
5mg/50mg/100mg/500mg
| 规格： | 5mg | 产品价格： | ￥100.0 |
|---|---|---|---|
| 规格： | 50mg | 产品价格： | ￥100.0 |
| 规格： | 100mg | 产品价格： | ￥100.0 |
| 规格： | 500mg | 产品价格： | ￥100.0 |
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文献和实验In vitro transcription with yeast nuclear extract
+ 0.1 M potassium acetate to each tube so that the final volume of the reaction will be 25 microliters (e.g. if 2.5 microliters of extract will be added, add 2.5 microliters HA + 0.1 M KOAc). Mix well and quick spin tubes. This addition of HA + 0.1M
In vitro transcription with yeast nuclear extract
on the volume of extract to be added to each reaction, add buffer HA + 0.1 M potassium acetate to each tube so that the final volume of the reaction will be 25 microliters (e.g. if 2.5 microliters of extract will be added, add 2.5 microliters HA + 0.1 M KOAc). Mix
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
bromophenyl blue [BioRad] (~0.1% in H2O)per ml of 5X buffer (this may inhibit the binding of some proteins )Store buffer at -70 degrees.--------------------------------------------------------------------------------Gel shift reactions are performed as follows
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