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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
咨询
- 英文名:
Tin(ii)sulfate
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
7488-55-3
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验Large scale nuclear extract preparation
pellets in nuclear lysis buffer (5ml/pellet).Perform one stroke with Dounce homogenizer 3 Add lysis buffer to 40ml and 2ml of 4M ammonium sulfate and agitate for 30-60min at 4℃ IV.Reprecipitate nuclear proteins 1.Centrifuge lysates in 55.2Ti rotor (35
Large scale nuclear extract preparat
buffer (5ml/pellet).Perform one stroke with Dounce homogenizer 3 Add lysis buffer to 40ml and 2ml of 4M ammonium sulfate and agitate for 30-60min at 4℃ IV.Reprecipitate nuclear proteins 1.Centrifuge lysates in 55.2Ti rotor (35,000rpm,60min,1℃ or 60Ti
96-well RNA In Situ Hybridization Protocol
: 4% paraformaldehyde/PBS) for 5 min.11. Fix 10 min. in 4% paraformaldehyde/PBS.12. Rinse 3X with PBS + 0.1% Tween 20 (PBT).13. Wash embryos 3X with PBT before hybridization.II. PREPARATION OF PROBEThe composition of the buffers used in sections II and III and general
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