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of PCR primer PE 1.0 (2X diluted with 1X TE, pH 7.4) 1 µL of PCR primer PE 2.0 (2X diluted with 1X TE, pH 7.4) ii. Denature at 98°C for 30 sec, followed by 18 amplification cycles (98°C, 10 sec; 65°C

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2.4 IPG干胶条再水化溶液... 31 2.4.1再水化液的组成... 31 2.4.2再水化液的准备... 34 2.5 MultiphorⅡ和Immobiline干胶条套件（Immobiline DryStrip Kit）... 34 2.5.1 IPG干胶条再水化——Immobiline干胶条再泡胀盘（Immobiline DryStrip Reswelling Tray） 34 2.5.2准备等电聚焦（IEF）... 36 2.5.3 样品杯加样... 38 2.5.4 纸桥

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and enzymes that harbors the ability torecognize and cleave foreign nucleic acids based on their sequencesignature. Three known CRISPR systems, types I, II, and III, havebeen described so far [ 12 – 15 ]. Among these different CRISPRsystems, type II CRISPR