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文献和实验Propagation, Quantification, Detection, and Storage of West Nile Virus
) 25% glycerol in TNE (v/v) (see recipe for TNE) TNE (see recipe ) T
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
of a standard or sample is calculated by subtracting the GIG value from the GAM value for each sample. Add 8.6 µl of (GIG, 77 mg/ml) and 10.0 µl (GAM, 66 mg/ml) to two separate tubes containing 10 ml COATING BUFFER. To a 96 well plate (Nunc), multipipet 100 µl
6. Centrifuge the block in the GPR centrifuge at 3000 rpm at 4degC for 30 minutes. 7. Carefully remove 200 ul of the supernatant from each well in the 96 well block with the 12 channel pipetter and transfer them to a v-bottom microtiter plate, being careful
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