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文献和实验Amino-allyl Reverse Transcription
with the amino-allyl dye coupling procedure all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cye dyes from coupling to free amine groups in solution. Fill one Microcon 30 concentrator with 450 ul water. Add neutralized
Revised amino-allyl labeling protocol
and the Howard Hughes Medical Institute http://cmgm.stanford.edu/pbrown/protocols/amino-allyl.htm A. RT Reaction 1. To anneal primer, mix 1-2 m g mRNA with 5 ug of anchored oligo-dT [(dT)20 -VN] (Operon, HPLC purified) in a total volume of 18 m L
Amino-allyl Reverse Transcription
Amino-allyl Reverse Transcription (this protocol was adapted by Joe DeRisi from one developed at Rosetta Inpharmatics) Resuspend the contents of one pack of the monofunctional NHS-ester Cy3 or Cy5 dye (mono-functional Cy3 or Cy
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