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- 2025年07月07日
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| 规格： | 5mg | 产品价格： | ￥100.0 |
|---|---|---|---|
| 规格： | 50mg | 产品价格： | ￥100.0 |
| 规格： | 100mg | 产品价格： | ￥100.0 |
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文献和实验Phycoerythrin conjugation protocol
concentrator (Centricon 30 or 100 are fine) Apply to top of separation column. Elute with PBS. Pump column at approximately 0.3 ml/min. Order of elution is conjugate first (MW ~400,000) then free PE (MW ~240,000) then free Ab (MW ~150,000
neuromelanin isolation and identification
7.5, were sonicated, and incubated for 3 h at 37o C in a shaking water bath. The preparations were centrifuged at 10,000 G at RT for 30 min and the supernatant removed. Two ml of 5 mg/ml SDS in 75 mM Tris, pH 7.5 with 0.33 mg/ml proteinase K (Sigma) was added
neuromelanin isolation and identification
, and incubated for 3 h at 37o C in a shaking water bath. The preparations were centrifuged at 10,000 G at RT for 30 min and the supernatant removed. Two ml of 5 mg/ml SDS in 75 mM Tris, pH 7.5 with 0.33 mg/ml proteinase K (Sigma) was added to the pellet
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