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文献和实验Sequential RNA and DNA fluorescence in situ hybridization
) and air dry; Denature a biotin-labeled RNA FISH probe for 10 minutes at 85 °C and keep at 37 °C for 30 minutes (note 4); Add the RNA FISH probe as a drop onto a microscope slide. Place the coverslip cell-side down on top of the RNA FISH probe
(or [alpha-32P]-dCTP)** (1.85MBq = 50µCi) 6µl Klenow Fragment, exo- (5u) 1µl 4.Shake the tube and spin down in a microcentrifuge for 3-5sec. Incubate for 10min at 37℃. 5.Add
线粒体荧光探针大全:TMRM,Mitotracker,JC-1
reticulum when used at higher concentrations (~5�50 µM or ~1�10 µg/mL). DiOC6 (3) (D273) stains mitochondria in live yeast 21,117�119 and other eukaryotic cells,98,120 as well as sarcoplasmic reticulum in beating heart cells.121 It has also been used
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