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无锡云萃生物
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| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
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文献和实验Phycoerythrin conjugation protocol
concentrator (Centricon 30 or 100 are fine) Apply to top of separation column. Elute with PBS. Pump column at approximately 0.3 ml/min. Order of elution is conjugate first (MW ~400,000) then free PE (MW ~240,000) then free Ab (MW ~150,000
DETECTION OF RECOMBINANT PROTEINS BY ELISA
, and read the next day. If no color develops after 1 hour, plates can be wrapped in foil and kept at 4°C overnight. Carbonate Coating Buffer, pH 9.6 (0.15 M sodium carbonate, 0.35 M sodium bicarbonate, 0.03 M sodium azide
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
. o Resuspend in 1ml of 1X Buffer A (TBS). o Transfer to 1.5ml tube. o Spin @10K, 1min, RT, aspirate supernatant. o Put tube with pellet on ice. o To store the pellets overnight, put tube in dry ice and store at -80&Mac251;C until ready
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