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文献和实验alkyne?azide cycloaddition (CuAAC) reaction has been used to synthesize cyclic mini?DNA duplexes. The reaction is carried out on 5??alkyne?3??azide?labeled hairpin loop oligonucleotides and proceeds in high yield under mild conditions in as little as 5
Natural Product Chemistry in Action: The Synthesis of Melatonin Metabolites K1 and K2
) ortho -lithiation-iodination with tert -butyllithium-1,2-iodoethane, (c) use of the Sonogashira reaction with N -acetyl propargylamine, and (d) sequential alkyne hydration/formylation to give (K1 ) or alkyne to give (K2 ).
Phycoerythrin conjugation protocol
concentrator (Centricon 30 or 100 are fine) Apply to top of separation column. Elute with PBS. Pump column at approximately 0.3 ml/min. Order of elution is conjugate first (MW ~400,000) then free PE (MW ~240,000) then free Ab (MW ~150,000
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