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文献和实验Glycol Methacrylate Embedding for Immunohistochemistry
, periodate/lysine/paraformaldehyde, and buffered paraformaldehyde solutions have all been used successfully. Try to keep the sample size small using 10mm x 10mm x 2 mm as a maximum. Fixation should be done for several hours at 4°C. Wash out the fixative
. Take the precipitate using a sterile transfer pipet (IWAKI 7801-002) (Do not suck! Wind the precipitate around the pipet.) or by centrifuging Dissolve the precipitate in (2/5 x X) ml TE containing 1 M NaCl and RNase A (1 to 10 ug/ml). Incubate at 55oC for 1 hr
Glycol Methacrylate Embedding for Immunohistochemistry
of the biological samples can be done in any way appropriate for the antigen you are seeking. Cold acetone, periodate/lysine/paraformaldehyde, and buffered paraformaldehyde solutions have all been used successfully. Try to keep the sample size small using 10mm x 10
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