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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
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- 英文名:
N
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
1049741-55-0
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
is incubated for 35 thermal cycles as follows: 95°C for 45 seconds 55°C for 30 seconds (lower temperature for primers less than 20nt) 72°C for 1 minute f. 75 µl of H20 is added to the reaction below the oil and 90 - 95 µl of the diluted reaction
Crystallization of Kinesin Family Motor Proteins
.0% PEG 8000, 0.8 M NaCl, 50 mM Na2HPO4/NaH2PO4 (pH 6.8) and 7 mM DTT at 18°CYun et al., 2003KIF1AVapor diffusion at 20°C for 24 hAMPPNP: 27% w/v PEG4000, 100 mM Tris-HCl pH 8.5, 200 mM sodium acetate and 3% w/v xylitol with a final concentration
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Buffer (Vial 6) Incubate at 65°C for 15-20 minutes Add probe mix to microarray and Hyb O/N at 55-65°C. Washes: 10'@55°C using 2XSSC, 0.2% SDS 10'@RT° using 2XSSC 10'@RT° using 0.2XSSC Image Arrays GENERAL
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