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文献和实验Syntheses of DNA Duplexes That Contain a N4C‐Alkyl‐N4C Interstrand Cross‐Link
a simple procedure for preparing short DNA duplexes that contain a single N4 C?alkyl?N4 C interstrand cross?link. The synthesis is carried out on an automated DNA synthesizer using standard phosphoramidite chemistry. The cross?link is introduced
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
containing the antibody/agarose complex. Mix by flicking tube gently. (36) Incubate at RT for 15 min. (37) Pellet agarose by brief centrifugation (3000-5000 x g (6600 rpm) for 1 min), and collect supernatant into new tubes. (38) Repeat steps 35
media: Ap 50 μg/ml, Km 50 μg/ml. For BACs and low copy vectors, on plates and in liquid media: chloramphenicol (Cm) 20 μg/ml, Ap 50 μg/ml and Km 15 μg/ml. Tetracycline (Tc) concentrations for selection of pSC101BADγβαA[tet] was 5 and 3 μg/ml on plates
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