Adaptable: Suited for any injection route to target any organ 适应性:适用于任何注射路径,针对任何器官
Safe: Maintain healthy animals and organs 安全:维护健康的动物和器官
Time-saving: Ready-to-use reagent with an easy protocol 节省时间:易于使用的试剂
Tailored: Customized protocols from in vivo delivery experts 量身定制:来自体内给药专家的定制方案
Overview(概述)
Specifications(规范)
Reagent 试剂
in vivo-jetRNA® transfection reagent
Molecule delivered 转染核酸
mRNA
Applications 应用
Vaccination / immunization Cancer therapy in vivo functional studies (Gene expression, CRISPR genome editing) 疫苗 / 免疫研究 肿瘤治疗 体内功能研究(基因表达,CRISPR基因编辑)
Targeted organs 靶向器官
All organs (depending on the injection route) 所有器官(参考注射途径)
Injection routes 注射途径
Various administration routes (systemic or local) 多种注射途径(全身及局部)
Number of injections in mice 试剂用量
1 mL is sufficient to perform at least 50 intravenous injections or 100 intramuscular injections 1 mL足够在小鼠身上做50次静脉注射或者100次肌肉注射
Storage 储存条件
5 °C ± 3°C, stable for at least one year when stored appropriately 5 °C ± 3°C
Provided with 配套提供
mRNA buffer mRNA 缓冲液
Summary(总结)
in vivo transfection reagents are the most powerful alternative to viral vectors for nucleic acid delivery. They are easy to use, cost-effective and considered as safe and efficient vehicles for RNA delivery. mRNA transfection is rapidly emerging as a promising method for nucleic acid-based therapy and offers an attractive substitute to plasmid DNA. Non-viral mRNA delivery methods have already proven their efficiency in vaccination through antigen presenting cells modification and in anti-cancer therapy by directly targeting malignant cells. The intrinsic advantage of mRNA-based immunotherapy relies on the self-adjuvant activity of mRNA and the fact that small amounts of encoded antigen are sufficient to obtain robust immune response.
in vivo-jetRNA® is a transfection reagent specifically developed to deliver mRNA in vivo. This reagent can be used to target multiple organs, by using systemic or local injection routes, in various animal models. mRNA delivery using in vivo-jetRNA® is user-friendly, with a simple 2-step protocol and can be used for vaccination purposes, anti-cancer studies, genome editing using CRISPR/Cas9 method or protein replacement. 在体内转染试剂是最强大的替代病毒载体的核酸传递。它们易于使用,具有成本效益,被认为是安全有效的RNA传递载体。mRNA转染是一种很有前途的核酸治疗方法,是质粒DNA的一种很有吸引力的替代品。非病毒mRNA传递方法已经证明了它们通过抗原提呈细胞修饰接种疫苗和直接靶向恶性细胞的抗癌治疗的有效性。基于mRNA的免疫治疗的内在优势依赖于mRNA的自辅助活性,以及少量编码抗原就足以获得强大的免疫应答。