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- 英文名:
2-Chloro-4-methylpyridine;
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
3678-62-4
- 规格:
10mg/50mg/100mg
| 规格: | 10mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
云萃
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer
(1,2-dioxetane-3,2'-(5'-chloro)tricyclo[3,3.1.13.7 ]decan}-4-yl) phenyl phosphate, more commonly known as CSPD (Cat. No. 1655 884). Blot Preparation: 1. Denature DNA in gel by soaking in 1X denaturation buffer 30-60 min. 2. Neutralize by soaking gel in 1X
). However, for localization of cells containing transduced lacZ, chromogenic substrates that yield a precipitated product are desirable (Holt and O'Sullivan, 1958, Pearse 1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro
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