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- 文献和实验
- 技术资料
- 保存条件:
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- 英文名:
(2S,3S)-3-(tert-Butoxycarbonylamino)-2-methyl-butanoic acid;
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
186494-09-7
- 规格:
10mg/50mg/100mg
| 规格: | 10mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
云萃
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
aggregation (darning) needle (DN-09, BLS Ltd., Hungary,) 70% ethanol. Method: Place few rows of KSOM microdrops (roughly 3 mm in diameter) into tissue culture dish using syringe (e.g. 3 drops in the first and fourth and 4-5 in the second
PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS
Grip 35 x 10 mm, Falcon 3001-3); KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410); aggregation (darning) needle ( DN-09, BLS Ltd., Hungary ,) 70% ethanol. Method: 1. Place few rows
-well plate; Roughly 3x106 J2-3T3s (a fully confluent T75?) 1.5ml 10x reconstitution buffer 1.5ml 10x DMEM 12ml rat tail type 1 collagen (>3.8mg/ml) 10N NaOH Glacial acetic acid (in case) Method 1. Pre-chill pipettes, keep collagen
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